灵芝保健品中三萜化合物HPLC分离条件的优化

[目的]优化灵芝中三萜化合物HPLC法的分离条件。[方法]用RP-HPLC法,考察不同提取剂、不同梯度洗脱、不同进样量对灵芝中三萜类化合物分离的影响,同时在最佳条件下分离测定4种市售保健品中三萜类组分。[结果]灵芝中三萜化合物提取剂为氯仿;优选出的灵芝三萜组分的HPLC分离条件为：流动相A为0.2%乙酸-水,流动相B为乙腈,梯度洗脱;检测波长为254nm;进样量为10μl;市售的4种灵芝保健品中只有2种保健品的HPLC分离图谱能与灵芝原药材对应。[结论]该试验得到分离灵芝中的三萜类组分的最佳分离条件,同时对4种市售保健品中三萜类组分进行了测定,此法可用于灵芝提取物的质量控制和其保健品的鉴定。

Optimization of Separation Condition of Triterpenoids from Ganoderma lucidum under HPLC

[Objective]The aim was to optimize the separation condition of triterpenoids from Ganoderma lucidum. [Method]The effects of different extraction solvents,different mobile phases and different injecting amount on the separation of triterpenoids were studied by RP-HPLC,and the triterpenoids of 4 kinds of health care products were determined under the optimum condition. [Result]The optimum condition were as follows：extraction solvents of chloroform,aqueous phase of 0.2% acetic acid-water,injecting amount of 10-30 ul,and the chromatogram of 2 kinds of the health care products were similar with that of Ganoderma lucidum medicinal material. [Conclusion]The optimum conditions were obtained,and the 4 kinds of health care products were determined by this method,which could used to control the extraction quality of Ganoderma lucidum and evaluate the health care products.