| 重组马槟榔甜蛋白MabinlinⅡ在大肠杆菌中的表达 |
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| 引用本文： | 顾文亮,夏启玉,胡新文,郭建春.重组马槟榔甜蛋白MabinlinⅡ在大肠杆菌中的表达[J].广西农业生物科学,2009(3):439-444. |
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| 作者姓名： | 顾文亮 夏启玉 胡新文 郭建春 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所;海南大学农学院; |
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| 基金项目： | 国家自然科学基金(30760025)资助 |
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| 摘 要： | MabinlinⅡ是我国所特有且唯一的植物甜蛋白，在体外至今没有得到具有甜味的基因表达产物。本文根据己知马槟榔甜蛋白的序列设计引物克隆MabinlinⅡ基因，对基因进行剪切重组。将重组基因克隆至大肠杆菌表达载体pET-30a（＋）中，构建了3个重组表达载体，转化大肠杆菌BL21（DE3），得到三株表达重组马槟榔甜蛋白的大肠杆菌工程菌。经IPTG诱导，3个重组MabinlinⅡ基因可在大肠杆菌BL21（DE3）中高效表达。
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| 关 键 词： | 马槟榔 甜蛋白 诱导表达 |
Expression of Recombinant Mabinlin Ⅱ in Escherichia coli Induced by IPTG |
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| Gu Wenliang , Xia Qiyu Hu Xinwen Guo Jianchun Institute of Tropical Bioscience , Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou, College of Agriculture,Hainan University.Expression of Recombinant Mabinlin Ⅱ in Escherichia coli Induced by IPTG[J].Journal of Guangxi Agricultural and Biological Science,2009(3):439-444. |
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| Authors: | Gu Wenliang Xia Qiyu Hu Xinwen Guo Jianchun Institute of Tropical Bioscience Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou College of Agriculture Hainan University |
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| Institution: | Gu Wenliang 1,2 Xia Qiyu 1 Hu Xinwen 2 Guo Jianchun 1 1 Institute of Tropical Bioscience , Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101,2 College of Agriculture,Hainan University,570228 |
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| Abstract: | Mabinlin Ⅱ is the unique plant sweet protein from Capparis masaikai which is only found in China but no sweetness gene expression products in vitro was reported so far. The recombinant Mabinlin Ⅱ genes were subcloned into a E. coli expression vector pET-30a（＋）. Three recombined plasmid pET-30a（＋） were then transformed into E. coli BL21 （DE3） host cells. Upon induction with IPTG, the recombinant Mabinlin Ⅱ protein were highly produced measured by SDS-PAGE. |
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| Keywords: | Capparis masaikai Sweet protein Inducing expression |
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