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| 离子束介导外源DNA转化小麦的当代生物效应分析 | |
| 引用本文： | ZHAO Xin-liang,赵新亮,王铁固,欧行奇,冯伟森,王卫东,秦广雍.离子束介导外源DNA转化小麦的当代生物效应分析[J].贵州农业科学,2009,37(6). |
| 作者姓名： | ZHAO Xin-liang 赵新亮 王铁固 欧行奇 冯伟森 王卫东 秦广雍 |
| 作者单位： | 1. 河南科技学院,生命科技学院,河南,新乡,453003 2. 洛阳市农业科学研究院,河南,洛阳,471002 3. 郑州大学,河南省离子束生物工程重点实验室,河南,郑州,450052 |
| 基金项目： | 农业部农业公益性行业科研专项经费项目 |
| 摘 要： | 为拓宽小麦种质资源,创造优异新种质,以6个玉米品种和1个银杏品种的总DNA为转化供体,以4个小麦品种为转化受体,进行了离子束介导遗传转化研究试验.结果表明,离子注入和离子束介导遗传转化后,受体材料田间出苗率有明显降低,离子注入、DNA溶液浸泡和离子束介导遗传转化处理小麦当代都有一定的变异率,具有明显的生物效应;离子注入和离子束介导遗传转化的当代生物效应二者间没有明显差异,但较DNA溶液浸泡均更明显.田间出苗率和当代变异率能较一致地反映离子束介导遗传转化存在基因型上的差异. |
| 关 键 词： | 小麦 离子束 外源DNA 遗传转化 生物效应 |
The Biological Effect of Generation by Exogenous DNA Transformation via Ion Beam Treatment in Wheat |
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| ZHAO Xin-liang,WANG Tie-gu,OU Xing-qi,FENG Wei-sen,WANG Wei-dong,QIN Guang-yong.The Biological Effect of Generation by Exogenous DNA Transformation via Ion Beam Treatment in Wheat[J].Guizhou Agricultural Sciences,2009,37(6). | |
| Authors: | ZHAO Xin-liang WANG Tie-gu OU Xing-qi FENG Wei-sen WANG Wei-dong QIN Guang-yong |
| Institution: | 1.School of Life Science and Technology;Henan Institute of Science and Technology;Xinxiang;Henan 453003;2.Luoyang Institute of Agricultural Science;Luoyang;Henan 471002;3.Henan Key Laboratory of Ion Beam Bio-engineering;Zhengzhou University;Zhengzhou;Henan 450052;China |
| Abstract: | Four wheat varieties as the transformation accepters,and six maize varieties and one gingko variety as the transformation donors were used in the test of genetic transformation via ion beam.The results showed that the seedling rate of the accepters treated by ion irradiation and genetic transformation via ion beam treatment decreased obviously and the wheat plants of M0 generation treated by ion irradiation,soaking DNA solution and genetic transformation via ion beam treatment have a certain variation rate ... |
| Keywords: | wheat ion beam exogenous DNA genetic transformation biological effect |
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