临界生长温度锻炼对棉苗抗冷性的影响

研究了棉苗经过不同低温(12℃、14℃、16℃)锻炼7 d后,对较长时间(7 d)的低温(4℃)胁迫的耐受能力,并探讨了与其相关的可能机理.结果表明:经不同低温锻炼7 d后,所有棉花品种的幼苗脯氨酸含量都有不同程度的增加,其中12℃临界生长温度锻炼后增幅最明显;临界生长温度(12℃)下锻炼后的棉苗能耐受较长时间(7 d...

Effect of Acclimating at the Critical Growth Temperature on Chilling Resistance of Cotton Seedlings

Chilling tolerance of cotton seedlings has been studied after acclimating at different low temperature（12℃、14℃、16℃） for 7 days and chilling stress at 4℃ for 7 days, and the possible mechanism has been discussed. The results showed that the content of proline increased in all treatments acclimating at different temperature for 7 days, among which the acclimating temperature at 12℃ was beneficial preferably, and the seedlings can tolerate chilling stress at 4℃ longer(7 days). The results approved that the chilling-resistance of cotton seedlings may be improved after acclimation at the survival threshold low temperature(12℃). Moreover, the soluble proteins in cotton seedlings were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The results of the analyzing by Tanon GIS showed that the content and types of protein had changed significantly, and the new low-temperature-induced proteins(namely anti-freeze protein) were discovered at 80 kd and 90 kd in cold-hardening cotton seedlings.