野生红花隔距兰根部内生真菌研究

为了解内生真菌在红花隔距兰根部的种类多样性以及受生境的影响,采用组织培养法,对海南霸王岭地区不同生境下的野生红花隔距兰根部进行内生真菌分离与鉴定.结果共分离到内生真菌145株,确定为15属,其中子囊菌门的Penicillium 16株,Aspergillus 12株、Pestalotiopsis5株、Fusarium 19株、Neonectria 2株、Chaetomium 4株、Calonectria 5株、Xylaria 5株,半知菌类的Paecilomyces 7株、Alternaria 2株、Phomopsis 8株、Colletotrichum 32株,接合菌门的Rhizopus5株、Mucor 7株,卵菌门的Albugo 6株,有10株菌经ITS序列比对分别与Botryosphaeria sp.、Beltraniella sp.相似度较低,有待进一步鉴定研究确定其分类地位.发现Colletotrichum sp.、Penicillium sp.、Fusarium sp.为优势真菌,生在于热带针叶林的植株根部分得的内生真菌最多,生物多样性最丰富.

Endophytic fungi in root of wild Cleisostoma williiamsonii

As an epiphytic orchid, Cleisostoma williiamsonii is a very beautiful ornamental plant, and plays an important role in tropical forest ecosystems. In this paper, tissue culture method was used to isolate endophyte in root of wild C. williiamsonii from Bawangling, Hainan. The total 145 isolates were identified into 15 genera, including Penicillium 16 strains, Aspergillus 12 strains, Pestalotiopsis 5 strains, Fusarium 19 strains, Neonectria 2 strains, Chaetomium 4 strains, Calonectria 5 strains, Xylaria 5 strains, Paecilomyces 7 strains, Alternaria 2 strains, Phomopsis 8 strains, Colletotrichum 32 strains, Rhizopus 5 strains, Mucor 7 strains; Albugo 6 strains. Acorrding to the ITS sequences, another 10 strains needed more identification, which had low degree of similarity with Botryosphaeria sp., Beltraniella sp.. It showed that Colletotrichum sp., Penicillium sp., Fusarium sp. were the predominant species, and had the richest biodiversity in tropical coniferous forests.