泥鳅属和副泥鳅属鱼类自交及杂交子一代生长性能及丙二醛含量的比较

以泥鳅属鱼类黑龙江泥鳅(Misgurnus mohoity,H)、北方泥鳅(Misgurnus bipartitus,N)、泥鳅(Misgurnus anguillicaudarus,二倍体B,四倍体T)和副泥鳅属鱼类大鳞副泥鳅(Paramisgurnus dabryanus,P)为亲本,进行自交与正反交,获得11种子代,它们分别为(雌×雄):H×H(HH)、N×N(NN)、P×P(PP)、B×B(BB)、P×B(PB)、N×H(NH)、N×P(NP)、P×H(PH)、B×P(BP)、T×P(TP)、P×T(PT)。经过270d的养殖试验,在60、90、120、150和270d这5个时间点测定了这11种子代的全长和体质量,同时在60、90和120d对丙二醛(MDA)含量进行了评估。结果表明:(1)NP在饲养试验中一直保持较好的生长性状;(2)PB与PP在60~90d的早期阶段,生长趋势较差,之后随着时间的推移开始上升,表现出较好的生长趋势,且这2种子代之间无显著性差异。PB在270d时体质量显著性高于其他子代;(3)NP在60d和90d所占全长和体质量最大20%的比例均高于其他子代。TP在270d时在全长和体质量最大和最小20%中出现频率都较高,而PB在所有采样时间点全长和体质量的最大和最小20%中的出现频率都较高;(4)具备优良生长性状的NP、PP及PB中,NP和PP在养殖过程中均具有较低的MDA含量,PB的MDA含量在第90天升高,然后长期处于较高水平。由此推断,NP和PP在生长和免疫机能上都具有优势;PB在生长性状上具有选育潜力。

Comparisons of growths and malonaldehyde contents of selfing/hybrid offsprings of Misgurnus and Paramisgurnus fish

Misgurnus mohoity (H),Misgurnus bipartitus (N),Misgurnus anguillicaudarus (diploid,B; tetraploid,T) and Paramisgurnus dabryanus (P) were used here to produce 11 kinds of selfing/hybrid offsprings,namely H×H (female×male,HH),N×N (NN),P×P (PP),B×B (BB),P×B (PB),N×H (NH),N×P (NP),P×H (PH),B×P(BP),T×P (TP),and P×T (PT).The culture experiment lasted for 270 d.Total lengths and body weights of the 11 different offsprings were measured at 60d,90d,120d,150d,and 270d,meanwhile the malonaldehyde (MDA) contents were measured at 60 d,90 d,and 120 d.Results obtained here showed that NP kept good growth performance in the entire experimental period.Growth tendencies of PB and PP were poor during the early period from 60d to 90 d,but they began to rise and became good as time went on.In addition,there was no significant difference in growth traits between PB and PP (P>0.05).The body weight of PB at 270 d obviously outweighed other offsprings (P<0.05).The ratios of NP in the 20% of the maximum total length and body weight at 60 d and 90 d exceeded other offsprings.The ratios of TP in the 20% of the maximum and minimum total length and body weight at 270 d were relatively high,while always high for PB in all the sampling times.Both NP and PP with good growth potential had relatively low MDA contents.MDA content of PB rose at 90 d and then stayed high.In conclusion,NP and PP had advantages in growth and immune performance,and PB presented breeding potential in growth traits.