低温处理对白三叶叶绿素、可溶性糖和丙二醛等的影响

为探索低温胁迫对白三叶的叶绿素、可溶性糖和丙二醛等生理指标的影响,为白三叶的抗寒性育种和资源的合理利用提供基础数据,试验以新品系08-2和海法白三叶(Trifolium repens L.‘haifa’)为材料,依次设置10℃、5℃、0℃、-5℃、-10℃、-15℃等6个处理,研究低温下叶绿素、可溶性糖和丙二醛等的变化,对材料的抗寒性进行评价。结果表明,变温对白三叶的叶绿素、可溶性糖和丙二醛等的影响较大。随着温度的降低,白三叶的叶绿素含量总体呈现降低的趋势,在较低(≤0℃)温度处理下,08-2的叶绿素含量降低速度小于海法的；08-2的可溶性糖含量在0℃时达到最高,海法的在5℃达到最高；丙二醛含量和电导率逐渐升高,但08-2变化幅度低于海法。综合分析表明,新品系08-2耐寒性优于海法白三叶。

Effects of Temperature Stress on Chlorophyll Content, Soluble Sugar and MDA in Trifolium repens L.

The objective of this study is to explore the effect of low temperature stress on chlorophyll, soluble sugar and MDA in Trifolium repens L.. The new strain‘08-2’and‘Haifa’of Trifolium repens were used as the materials, and then the treatments of 10, 5, 0, -5, -10, -15℃ were set. The variations of chlorophyll, soluble sugar and MDA were studied under low temperatures, and the cold resistances of the materials were evaluated. The results showed that varying temperature had a great influence on chlorophyll, soluble sugar and MDA in Trifolium repens L.. With the decrease of temperature, the chlorophyll content declined gradually. The declining speed of‘08-2’was less than that of‘Haifa’under lower temperature (≤0℃). The soluble sugar content of‘08-2’was the highest at 0℃, and the soluble sugar content of‘Haifa’was the highest at 5℃. With the decrease of temperature, the MDA content and electric conductivity increased gradually, but the change range of‘08-2’was lower than that of‘Haifa’. Comprehensive analysis showed that the cold resistance of the new strain‘08-2’was better than that of‘Haifa’.