| 一株家蚕病原物拮抗细菌的分离与鉴定 |
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| 引用本文： | 刘训理,孙长坡,马迎飞,王超,刘开启. 一株家蚕病原物拮抗细菌的分离与鉴定[J]. 蚕业科学, 2004, 30(3): 273-276 |
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| 作者姓名： | 刘训理 孙长坡 马迎飞 王超 刘开启 |
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| 作者单位： | 山东农业大学林学院,泰安,271018;仲恺农业技术学院 |
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| 摘 要： | 从泰山土壤中分离获得 1株对多种家蚕病原细菌和真菌具有强烈拮抗作用的细菌。经培养特征观察、生理生化指标测定、DNAG +Cmo1%值测定、16SrDNA碱基序列测定和同源性分析 ,鉴定该菌株属于类芽孢杆菌 ,定名为PaenibacillusCp-S316。该菌株已在GeneBank注册 ,注册号为AY2 92 989。初步研究发现 ,该菌株对家蚕生长发育无不良影响 ,发酵性状优异 ,抗菌物质耐高温 ,对盐酸和硫酸稳定 ,易于提取分离 ,具有一定开发价值。
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| 关 键 词： | 蚕病 拮抗菌 Paenibacillus Cp-S316 分离 鉴定 |
| 文章编号： | 0257-4799(2004)03-0273-04 |
| 修稿时间： | 2004-03-12 |
Isolation and Identification of An Antagonistic Bacteria Strain Against Silkworm Pathogens |
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| LIU Xunli SUN Changpo MA Yingfei WANG Chao LIU Kaiqi. Isolation and Identification of An Antagonistic Bacteria Strain Against Silkworm Pathogens[J]. Acta Sericologica Sinica, 2004, 30(3): 273-276 |
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| Authors: | LIU Xunli SUN Changpo MA Yingfei WANG Chao LIU Kaiqi |
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| Affiliation: | LIU Xunli 1 SUN Changpo 1 MA Yingfei 1 WANG Chao 1 LIU Kaiqi 2* |
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| Abstract: | One antagonist isolated from Tai Mountain soil was greatly resistant to various pathogenic bacteria and fungus of silkworm. By observation of the culture characters, measurement of the biochemistrical features and the values of DNA G+C mo1% and analysis of 16S rDNA sequences homology, the strain was identified as Paenibacillus sp and named as P sp Cp -S316 which was registered in GenBank on NCBI and the accession number was AY292989. The preliminary research found that the strain has no bad effects on growth and development of the silkworm and good fermenting characters,and that the antibiotic substance can survive at high temperature, hydrochloric acid and sulpluric acid and be easily extracted, which show the developing value of the strain. |
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| Keywords: | Silkworm diseaseAntagonists Paenibacillus Cp -S316IsolationIdentification |
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