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| 与桃果实非酸/酸性状连锁AFLP特异片段的克隆及序列分析 | |
| 引用本文： | 吴俊,束怀瑞,张学宁,张开春,姜立杰. 与桃果实非酸/酸性状连锁AFLP特异片段的克隆及序列分析[J]. 果树学报, 2004, 21(6): 526-529 |
| 作者姓名： | 吴俊 束怀瑞 张学宁 张开春 姜立杰 |
| 作者单位： | 1. 山东农业大学园艺学院,山东,泰安,271018;南京农业大学园艺学院,江苏,南京,210095 2. 山东农业大学园艺学院,山东,泰安,271018 3. 北京市农林科学院林业果树研究所,北京,100093 |
| 基金项目： | 北京科技合同项目(954830300)，北京市新星项目(953812200)。 |
| 摘 要： | 利用AFLP技术从京玉、美味正反交F1代69株群体中筛选到3个特异扩增片段,与桃果实非酸/酸性状紧密连锁。回收特异片段并将其克隆、测序,结果表明特异片段A、B、C均来自于EcoRI与MseI两内切酶酶切片段,大小依次为140、199、408bp,利用序列分析软件推测片段B、C中可能含有与糖水平调控作用相关的因子。上述结果对AFLP标记向SCAR标记的转换具有重要意义。 |
| 关 键 词： | 桃 非酸/酸 AFLP 克隆 序列 |
| 文章编号： | 1009-9980(2004)06-526-04 |
| 修稿时间： | 2004-04-27 |
Cloning and Sequence Analysis of AFLP Specific Fragment Related to Fruit Non-acid/acid Trait of Peach |
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| Wu Jun,Shu Huairui,Zhang Xuening,Zhang Kaichun,Jiang Lijie. Cloning and Sequence Analysis of AFLP Specific Fragment Related to Fruit Non-acid/acid Trait of Peach[J]. Journal of Fruit Science, 2004, 21(6): 526-529 | |
| Authors: | Wu Jun Shu Huairui Zhang Xuening Zhang Kaichun Jiang Lijie |
| Affiliation: | Wu Jun1,3,Shu Huairui1,Zhang Xuening2,Zhang Kaichun2,and Jiang Lijie2 |
| Abstract: | Three specific amplified DNA fragments linked to fruit non-acid /acid trait of peach were obtained from 69 F1 seedlings of the intra-specific cross between cultivars 'Jingyu' and 'Flavortop' by using AFLP technique. After cloning the specific fragments and sequencing,we found that fragment A,B and C all were digested by both EcoRⅠand MseⅠ,and were 140 bp,199 bp,and 408 bp in length respectively. It was presumed that fragments B and C might contain elements which are relevant to regulating the sugar content by utilizing sequence analysis software. This study would be important for further SCAR marker conversion. |
| Keywords: | Peach Non-acid/acid AFLP Cloning Sequence |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |