| 种子胚特异性启动子的克隆及其功能验证 |
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| 引用本文： | 巩艳青,夏阳.种子胚特异性启动子的克隆及其功能验证[J].山东林业科技,2007(3):9-12. |
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| 作者姓名： | 巩艳青 夏阳 |
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| 作者单位： | 山东农业大学园艺科学与工程学院,泰安,271018;山东省林业科学研究院 |
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| 摘 要： | 以水稻品种日本晴DNA为模板,用PCR的方法从水稻胚特异性表达基因OsESG上游序列扩增出特异性条带,克隆出种子胚特异性启动子,长度为1.1kb,且已知的功能部位序列没有发生改变。用它构建了带动报告基因GUS表达的双元载体,并用农杆菌介导法转化水稻得到了转基因植株。对GUS基因的表达检测表明,由该启动子序列引导的GUS基因仅在胚中特异性表达,而其它组织中都未表达,证实该启动子具有胚特异性表达的功能。
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| 关 键 词： | 水稻 胚特异性启动子 报告基因GUS 转基因 |
| 文章编号： | 1002-2724（2007）03-0009-04 |
| 收稿时间： | 2007-03-15 |
| 修稿时间： | 2007-03-15 |
Isolation and characterization of an embryo-specific promoter |
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| Gong Yanqing,Xia Yang.Isolation and characterization of an embryo-specific promoter[J].Journal of Shandong Forestry Science and Technology,2007(3):9-12. |
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| Authors: | Gong Yanqing Xia Yang |
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| Institution: | 1. College of Horticulture Science and Engineering, Shan Dong Agricultural University, Tai an 271018;2. Shangdong provincial Research Academy of Forestry |
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| Abstract: | A pair of specific primer was designed according to the sequence of the 5'upstream sequence of rice embryo-specific gene OsESG.A 1.1kb sequence was amplified from genomic DNA template of rice(Oryza sativa L.) by PCR.Sequence analysis showed no difference was found in known functiona1 regions.The cloned promoter has been used in construction of Agrobacterium binary vectors with GUS reporter gene,and severa1 transformed plants were obtained through Agrobacterium-mediated transformation.GUS activity in various tissues of transformed plants was examined and the results showed that GUS gene directed by the promoter sequence of OsESG was tissue-specifically expressed in embryo,in contrast to the expression pattern of GUS gene directed by 35S promoter,which was expressed in all the tissues tested. |
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| Keywords: | Oryza sativa Embryo-specific promoter Reporter gene GUS Transformation |
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