Study on the TLC Identification of Effective Components in the Sihuangzhili Granules

In order to establish and perfect the quality standard of Sihuangzhili granules, this experiment was conducted to identify coptidis rhizome, cortex phellodendri, rhubarb, scutellaria baicalensis, and radix isatidis in Sihuangzhili granules. We used TLC identification method to investigate the factors such as the developing agent, the expansion temperature, the expansion of the humidity, the source of the thin layer, the batch number and the color developing conditions.In the experiment, the mixture of cyclohexane, ethyl acetate, isopropyl alcohol, methanol, water, and triethylamine (3∶3.5∶1∶1.5∶0.5∶1) as the developing agent, was put into the expansion cylinder with the pre-saturated ammonia vapor, and was used to identify coptidis rhizome and cortex phellodendri under the UV lamp(365 nm);The mixture of ethyl acetate, butanone, formic acid and water (5∶3∶1∶1) as developing agent, sprayed with 2% ethanol solution of ferric chloride, was used to identify scutellaria baicalensis;At last, n-butanol, acetic acid, and water (19∶5∶5) as developing agent, dried and sprayed with indene ketone solution, heated at 105 ℃, was used to identify radix isatidis. The test results showed that under the test condition, the target spot was clear, with no tail, and the experiments were specifically suited in this test with strong specificity and good reproducibility.