| 甜瓜白粉病抗病基因的定位及候选基因分析 |
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| 引用本文： | 李冰,赵玉龙,朱强龙,张志鹏,樊超,栾非时,高鹏. 甜瓜白粉病抗病基因的定位及候选基因分析[J]. 中国蔬菜, 2017, 1(6): 17-24 |
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| 作者姓名： | 李冰 赵玉龙 朱强龙 张志鹏 樊超 栾非时 高鹏 |
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| 作者单位： | 1.农业部东北地区园艺作物生物学与种质创制重点实验室，黑龙江哈尔滨 150030；;2.东北农业大学园艺园林学院，黑龙江哈尔滨 150030；;3.黑龙江省农业科学院海南基地，海南三亚 572000 |
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| 基金项目： | 国家自然科学基金项目（31301791，31672177），东北农业大学“学术骨干”计划项目（16XG06），东北农业大学“青年才俊”计划项目（14QC09），国家西甜瓜产业技术体系- 分子育种岗位项目（CARS-26-02） |
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| 摘 要： | 以甜瓜高抗白粉病自交系MR-1为母本,易感白粉病自交系Top Mark为父本,构建BC_1P_2和F_2群体,经13个国际通用的甜瓜白粉病生理小种鉴别寄主鉴定,2015年东北农业大学设施园艺工程中心的白粉病发病病菌为P.xanthii生理小种1,甜瓜MR-1对P.xanthii生理小种1的抗性由单显性基因控制。通过对266个F2分离群体的抗病性鉴定和CAPS标记分析,采用复合区间作图法对甜瓜抗白粉病性状进行QTL分析,最终构建了1张包含203个CAPS标记的甜瓜遗传连锁图谱,并将抗病基因PXR定位在第12号染色体上M12-GH和M12-TE 2个标记之间,该基因与两侧翼标记间的距离分别为0.63 c M和0.42 c M,两侧翼标记间在甜瓜参考基因组上对应的物理距离为303 kb,该区域内含有60个预测基因,其中10个基因含有非同义单碱基突变。10个基因荧光定量分析结果显示,在抗病与感病甜瓜表达量存在较大差异的4个基因MELO3C002434、MELO3C002437、MELO3C002441、MELO3C002457为甜瓜白粉病抗病候选基因。
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| 关 键 词： | 甜瓜 白粉病 CAPS 抗病基因 |
Melon Powdery Mildew Resistance Gene Location and Candidate Gene Analysis |
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| LI Bing,ZHAO Yu-long,ZHU Qiang-long,ZHANG Zhi-peng,FAN Chao,LUAN Fei-shi,GAO Peng. Melon Powdery Mildew Resistance Gene Location and Candidate Gene Analysis[J]. China Vegetables, 2017, 1(6): 17-24 |
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| Authors: | LI Bing ZHAO Yu-long ZHU Qiang-long ZHANG Zhi-peng FAN Chao LUAN Fei-shi GAO Peng |
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| Affiliation: | 1.Key Laboratory of Biology and Genetic Improvement of Horticultural Crops（Northeast Region），Ministry of Agriculture，Harbin 150030，Heilongjiang，China；;2.Horticulture and Landscape Architecture College of Northeast Agricultural University，Harbin 150030，Heilongjiang，China；;3. Hainan Base of Heilongjiang Academy of Agricultural Sciences，Sanya 572000，Hainan，China |
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| Abstract: | BC1P2 and F2 generations were constructed by crossing a melon inbred line‘ MR-1’ highlyresistant to powdery mildew（Podosphaera xanthii）as female parent，and a melon inbred line‘ Top Mark’ highly susceptible to powdery mildew as male parent. The race of P. xanthii was identified as race 1 according to the infecting action of 13 international differential hosts for melon powdery mildew，and the resistance to powdery mildew was controlled by a single dominant gene based on the population genetic analysis of BC1P2 and F2. Through evaluating the resistance in 266 individuals of F2，the genotype analysis using cleaved amplified polymorphic sequences（CAPS），and quantitative trait loci（QTL）analysis using composite interval mapping method（CIM）for locating the locus resistant to powdery mildew，a genetic linkage map of melon contained 203 CAPS was constructed. One QTL named PXR was detected on chromosome 12 between the 2 CAPS markers，M12-GH and M12-TE，which were tightly linked to the gene resistant to powdery mildew with the genetic distances of 0.63 cM and 0.42 cM，respectively. Sixty putative genes were located in the region with the length of 303 kb between the 2 flanking markers. Ten of them with non-synonymous single nucleotide polymorphism（nsSNP） were further identified as candidate genes resistant to powdery mildew in melon. The results suggested that 4 genes （MELO3C002434，MELO3C002437，MELO3C002441，and MELO3C002457）showed bigger differences in expression of disease resistance and susceptible might be the candidate genes resistant to powdery mildew in melon. |
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| Keywords: | Melon Powdery mildew CAPS Disease resistance gene |
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