Expression of miRNA-93 in acute lymphocytic leukemia |
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Authors: | ZHANG Guan-ting WANG Bao-feng |
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Institution: | Department of Clinical Laboratory, The Affiliated Hospital of Henan Province Chinese Medicine Institute, Zhengzhou 450004, China |
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Abstract: | AIM: To investigate the expression of microRNA (miRNA)-93 in acute lymphocytic leukemia (ALL) and its effect on the proliferation of acute T-cell leukemia Jurkat cells.METHODS: The expression of miRNA-93 in the bone marrow samples of patients with ALL was measured by real-time PCR. After down-regulation of miRNA-93 by transfection with miRNA-93 inhibitor in the Jurkat cells, the cell viability, cell proliferation and cell cycle distribution were detected by CCK-8 assay, EdU assay and flow cytometry, respectively. Furthermore, the protein levels of cell cycle-related molecules such as cyclin D1, cyclin-dependent kinase 4 (CDK4), phosphorylation retinoblastoma (Rb) and P27 were measured by Western blot.RESULTS: miRNA-93 was highly expressed in the patients with ALL, and the expression level was highest in the high risk patients. Down-regulation of miRNA-93 inhibited Jurkat cell viability, arrested cell cycle in G1/S transition. In addition, the protein levels of cyclin D1, CDK4 and p-Rb were significantly decreased, the protein expression of P27 was increased in Jurkat cells trasfected with miRNA-93 inhibitor.CONCLUSION: miRNA-93 expression is increased in ALL patients. Down-regulation of miRNA-93 restrains cell proliferation in the acute T cell leukemia cell line Jurkat via regulating cell cycle-related molecules. |
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Keywords: | MicroRNA-93 Acute lymphocytic leukemia Cell proliferation |
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