Cloning and Bioinformatics Analysis of L2R Gene of Sheeppox Virus GY Strain

To explore the molecular characteristics of protein L2R from sheeppox virus(SPPV), genomic DNA was extracted from SPPV GY strain. The specific primers were designed and used to amplify the L2R gene from the genomic DNA by PCR. Then the PCR product was ligated into pGEM-T Easy vector. After transformation into E. coli Trans 5α, the positive clones were sequenced and the sequences were analyzed by the bioinformatic softwares. The result showed that L2R gene sequence contained an open reading frame (ORF) of 279 nucleotides and deduced protein consisted of 92 amino acids with the theoretical molecular weight of 10.92 ku and isoelectric point was 6.56. Analysis of secondary structure of protein L2R revealed that α-helix, β-strand, random coil and extended strand were 69.57%,9.78%,8.70% and 11.96%,respectively. Analysis of multiple sequence alignment showed that L2R gene from different capripox virus isolates were highly conserved, phylogenetic analysis showed that GY and NK, TU and SA was in a branch, indicating with a close genetic relationship among them. The present results laid a foundation for further studies of biological functions of protein L2R and interaction among the early proteins of capripox virus.