Effects of Polysaccharides from Ferula sinkiangensis Root on Proliferation of Chicken Peripheral Lymphocyte and Spleen Lymphocyte in vitro

To investigate the effects of Ferula sinkiangensis polysaccharides (FSP) on cell proliferation of chicken splenic lymphocytes and peripheral lymphocyte in vitro. By the method of water extract-alcohol precipitation,total Ferula sinkiangensis polysaccharide (FSP_t) and 30%,50%,70% gradient alcohol precipitation of polysaccharides (FSP₃₀,FSP₅₀ and FSP₇₀) were extracted, and detected by phenol-sulfuric acid method and infrared spectroscopy method. The safe concentration of all classifications of polysaccharide were detected and the effect of FSP on lymphocyte proliferation of chicken spleen and peripheral blood were measured by MTT method. The results showed that the absorption peak of FSP were conformed to belong by polysaccharide compounds. Peripheral blood T lymphocyte proliferation test indicated that FSP_t,FSP₃₀ and FSP₇₀ alone or cooperated with PHA could stimulate T lymphocyte proliferation under a certain concentration,and FSP_t showed a better proliferation effect. Spleen B lymphocyte proliferation test showed that FSP_t,FSP₃₀ and FSP₇₀ alone or cooperated with LPS could stimulate B lymphocyte proliferation under a certain concentration,FSP_t and FSP₇₀ showed a better proliferation effect. In conclusion,FSP showed the strong effect on lymphocyte proliferation of chicken spleen and peripheral blood in vitro,and that would be the materials for further research.