芥蓝耐热性鉴定及耐热转录因子MBF1c表达分析

芥蓝喜凉性气候,耐高温能力较弱,夏秋季栽培高温逆境往往使其生长发育不良,影响其产量、品质。本试验采用人工气候箱高温处理的方法,测定10份芥蓝材料在不同高温胁迫下的生长指标和生理生化指标,分析10份芥蓝材料在不同高温胁迫下各项指标的相对变化率。结果表明:高温胁迫下芥蓝的热害指数与生长指标(地上部鲜质量、地下部鲜质量、节间长)可以简单、较好地反映出芥蓝材料间耐热性差异;37℃/25℃、40℃/32℃处理的生理生化指标相对变化率能较好地反映出芥蓝材料间的耐热性差异;高温能诱导MBF1c转录因子的表达,使其表达量升高,且耐热性强的材料相对表达量较高,该转录因子的表达可能对芥蓝耐热性起到一个正调控作用。综合评价结果表明,矮脚香菇在这10份芥蓝材料中耐热性最强。

Identification of Heat Tolerance in Chinese Kale and the Expression Analysis of Heat Tolerance Transcription Factor MBF1c

Chinese kale（Brassica alboglabra L. H. Bailey）is a thermophilic vegetable，which can’t well grow at high temperature．High temperaturewould prevent Chinese kale from growing and result in poor quality of its seedlings，seriously influence yield and quality in later period in summer and autumn．Taking 10 Chisnses kale lines as experimental materials，the growth indexs and physiology indexs under different high temperature stress were measured．The relative change rate of 10 Chinese kale lines under different high temperature stress was analyzed．The results showed that heat damage index and relative growth could be used to determine the heattolerance ability of Chinese kale lines，which can be most quick and convenient．The difference in heat tolerance among the varieties could be indicated by physiological and chemical index under 37℃ /25 ℃ or 40℃ /32 ℃． After the treatment of 37 ℃ for 1 hour the expression variation of MBF1c was increased．According to the comprehensive analysis，‘Aijiaoxianggu’ was the most heat-tolerant lines.