Isolation,Identification and Pathogenicity Analysis of Morganella morganii in Clariasfuscus |
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| Authors: | WEI Yang-dao YI Yi LI Ya SHI Zheng-yu WU Shi-hua LIANG Jian-rong |
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| Affiliation: | 1. College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, China;2. Guangxi Key Laboratory of Green Processing of Sugar Resources, Guangxi University of Science and Technology, Liuzhou 545006, China;3. Key Laboratory for Processing of Sugar Resources of Guangxi Higher Education Institutes, Guangxi University of Science and Technology, Liuzhou 545006, China;4. Liuzhou City Jiale Fish Feed Limited Company, Liuzhou 545002, China |
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| Abstract: | In this study, we isolated a strain from diseased Clariasfuscus. Through morphological observation, physiological and biochemical tests, 16S rDNA and phylogenetic tree analysis method, it was concluded that the pathogen was Morganella morganii. Artificial infection experiment results showed that the pathogen had strong pathogenicity to Clariasfuscus, and also had strong pathogenicity to totilapia, loach and carp.The drug sensitivity test results showed that the KD01 strains had high resistance to 6 medicines, including penicillin G, ampicillin and novobiocin, and so on. And it had the sensitivity to the 15 medicines, including chemitrim, enrofloxacin and ciprofloxacin, and so on.The test results of hemorrhage of intestinal necrosis disease study provided a strong basis for the study of Clariasfuscus, also could guide farmers to the rational use of drugs. |
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| Keywords: | Clariasfuscus Morganella morganii 16S rDNA isolation and identification |
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