| Abstract: | In this study,to investigate the regulatory role of methyltransferase-like protein 3 (METTL3) on milk protein and fat synthesis in bovine mammary epithelial cells (BMEC),different BMEC models were established, the expression of related genes and localization of METTL3 were detected,and the impact of METTL3 on milk protein and fat synthesis in BMEC after adding β-estrogen (E),methionine (Met),and overexpression or inhibition of METTL3 were measured using Western blotting and imunofluorescience methods. The results showed that when E and Met were added or the METTL3 expression was promoted,the expressions of mTOR,p-mTOR,GlyRS,p-GlyRS,CSN2 and SREBP-1c were increased,oppositely,the gene expression were decreased. Those results indicated that METTL3 could affect the milk fat and protein synthesis by adjusting the expression of related signaling pathway gene.METTL3 was one of the important regulating milk synthetic molecules and had an positively regulation on milk fat and protein synthesis in cells. |
