基于ITS序列对林芝市售七种野生大型食用真菌的鉴定

以西藏林芝市售7种野生大型真菌(分别编号为XZQGJ、XZDJG、NCSBJ、NCNGJ、NCTLJ、NCSR、NCWZNGJ)为试材,采用分子生物学的方法对其进行鉴定,为进一步开发利用林芝地区大型真菌资源提供了参考依据。结果表明:XZQGJ属于腊伞属的Hygrophorus russula(红菇腊伞);XZDJG属于肉齿菌的Sarcodon cf.leucopus(裂盖肉齿菌);NCSBJ属于枝瑚菌属,与Ramaria sp.(小刺枝瑚菌)(KY774253.1)亲缘性最近,但林芝扫把菌与其它相似种均有一定的遗传距离,因此林芝扫把菌是一个独立的种;NCNGJ属于牛肝菌属,与Boletus recapitulatus(KP938967.1)亲缘性最近;NCTLJ属于钉菇属,与Gomphus clavatus(棒状钉菇)(KX008988.1)亲缘性最近;NCSR属于口蘑属,与Tricholoma matsutake(松口蘑)(MF521899.1)亲缘性最近,但林芝松茸与其它相似种均有一定的遗传距离,因此林芝松茸是一个独立的种;NCWZNGJ属于牛肝菌科的uncultured Bole...

Identification Research of Seven Wild Macrofungus Based on ITS Sequences

Seven types of wild macrofungi(numbered XZQGJ,XZDJG,NCSBJ,NCNGJ,NCTLJ,NCSR,NCWZNGJ) from Nyingchi in Tibet were used as test materials,which were identified by morphology.The results showed that XZQGJ belonged to Hygrophorus russula;XZDJG belonged to Sarcodon cf.leucopus;NCSBJ belonged to Mycobacterium,and Ramaria sp.(KY774253.1)had the closest affinity,but NCSBJ had a certain genetic distance from other similar species,so NCSBJ was an independent species;NCNGJ belonged to to the genus Boletus and was closest to Boletus recapitulatus(KP938967.1);NCTLJ belonged to the genus Flammulina and was closest to Gomphus clavatus(KX008988.1);NCSR belonged to Tricholoma and was related to Tricholoma matsutake(MF521899.1).Lingzhi Matsutake had a certain genetic distance from other similar species,so NCSR was an independent species;NCWZNGJ belonged to the uncultured Boletaceae.The results showed a theoretical basis for further development and their application value of wild macrofungi resources in Nyingchi.