Determination of Acetaminophen in Bactrian Camel Plasma by RP-HPLC

To establish the reversed phase high-performance liquid chromatography (RP-HPLC) method for determination of plasma concentration of acetaminophen in Bactrian camel, the chromatography was carried on a C18 column (150 mm×4.6 mm,5 μm).The mobile phase was composed of methanol-water (20:80),the flow rate was 0.5 mL/min,and the detection wavelength was set at 248 nm for acetaminophen.The retention time of acetaminophen was 6.5 min, and was not disturbed by other miscellaneous peaks.The calibration curve was linearity in the range of 0.08 to 10.24 μg/mL (R²=0.9997),and the quantitation limit of acetaminophen was 0.10 μg/mL.Both the inter-day and intra-day RSD were under 7.0%, the absolute recovery of the method was 80.39% to 93.56%,the relative recovery of the method was 94.66% to 104.73%.Therefore, the established method was simple, reproducible and accurate,which could be used for determination of plasma acetaminophen concentration and in vivo activities of CYP1A enzyme in Bactrian camel.