Genetic Diversity and Phylogenetic Relationship of Introduced Alpaca in Xinjiang

In order to understand the genetic diversity and phylogenetic relationship of the alpacas,39 blood samples of alpaca were collected in 3 places of Xinjiang (Qinghe county of Aletai city,Tacheng city and Tianshan wildlife park) and mitochondrial D-loop gene was amplified and sequenced. The results showed that 733 bp fragment was obtained. The population haplotype diversity (Hd),nucleotide diversity (Pi) and gene flow (Nm) was 0.954,0.0091 and 1.97,respectively. 25 haplotypes were defined among the 39 gene sequences,and H7 was the most shared haplotype by groups. The genetic distance of three alpaca groups was small (0.002 to 0.003). The genetic distance between alpaca and Lama glama,Lama guanicoe and Vicugna was 0.002,0.004 and 0.017,respectively. The genetic distance between alpaca and Camelus bactrianus was the highest (0.050 to 0.051).The results of cluster analysis showed that three alpaca groups were gathered first,and then gathered with Lama glama,Lama guanicoe and Vicugna, Camelus bactrianus belonged to another branch along. In conlusion,the genetic diversity and gene flow level of alpacas in Xinjiang was high and the genetic differentiation was relatively small. The genetic relationships between alpaca and Lama glama,Lama guanicoe and Vicugna were close, that with Camelus bactrianus was relatively far.