Effect of ClC-3 siRNA on cell cycle of HeLa cells |
| |
Authors: | YE Dong XING De-gang ZENG Zhi CHEN Li-xin WANG Li-wei |
| |
Institution: | 1. Department of Physiology, Basic College, Guangdong Pharmaceutical University, Guangzhou 510006, China;
2. Department of Pharmacology, Jinan University, Guangzhou 510632, China;
3. Department of Physiology, Medical College, Jinan University, Guangzhou 510632, China |
| |
Abstract: | AIM: To investigate the roles of ClC-3 chloride channels in the regulation of cell cycle and the relationship between ClC-3 chloride channels and the cell cycle regulators, such as cyclin D1, cyclin-dependent kinase (CDK)4, CDK6, P21 and P27 in the HeLa cells.METHODS: ClC-3 genes were silenced by the siRNA technique in the HeLa cells. The transfection efficiency of ClC-3 siRNA was detected by real-time PCR. The cell cycle distribution was analyzed by the flow cytometry. The protein expression of ClC-3, P21, P27, CDK4, CDK6 and cyclin D1 was determined by Western blot.RESULTS: ClC-3 was knocked down by ClC-3 siRNA in the HeLa cells. Transfection of the cells with ClC-3 siRNA arrested the cells at G0/G1 phases, decreased the expression of cyclin D1, CDK4 and CDK6, and increased the expression of P21 and P27.CONCLUSION: ClC-3 plays an important role in the cell cycle of HeLa cells through the G1-S transition point. ClC-3 may regulate the cell cycle progression by up-regulation of cyclin D1, CDK4 and CDK6 expression and/or by down-regulation of P21 and P27 expression. |
| |
Keywords: | ClC-3 chloride channels HeLa cells Cell cycle Cyclin-dependent kinase P21 P27 |
|
| 点击此处可从《园艺学报》浏览原始摘要信息 |
| 点击此处可从《园艺学报》下载免费的PDF全文 |
|