Research Progress on Novel Peptide Epitopes Technology

As an important component of the antigen, peptide epitopes can be recognized by T cell receptor (TCR) and antibodies which induced protective immunity by the host immune system. Traditional research methods, such as overlapping peptide synthesis is long time-consuming which greatly limits the study of epitopes. With the development of bioinformatics, high-throughput sequencing, CRISPR/Cas9 technology, MS and other technologies and explaining the processing mechanism of TAP-dificient protein constantly, the research for epitopes is being perfect continually. In this article, some new methods to study epitopes were introduced, including computer prediction of B cell or T cell epitopes, identification of epitopes based on the structure and interaction of MHC and antigenic peptides, screening epitopes by means of high throughput sequencing, screening epitopes by means of newly genome editing, the up-to-date research in TAP-deficient T cell epitopes, etc. Finally,future prospect for research direction of peptides was further forecasted.