Establishment and Preliminary Application of Nano-PCR and LAMP Methods for Bovine Parainfluenza Type-3 Virus |
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| Authors: | LI Jian-you LI Jia-wei WANG Chao GUO Li HE Hong-bin |
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| Institution: | 1. Northeast Agricultural University, Harbin 150030, China;
2. Key Laboratory of Special Animal Epidemic Disease, Ministry of Agricultural, Institute of Special Economic Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China;
3. Antu Animal Husbandry and Veterinary Station, Antu 133600, China |
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| Abstract: | This study was aimed to establish Nano-PCR and LAMP which were new rapid type of molecular detection technologies of bovine parainfluenza type-3 virus (BPIV3).The comparative specificity and sensitivity of BPIV3 Nano-PCR and LAMP PCR were tested, and the assay was applied to detect 10 clinical samples. The specificity and sensitivity tests showed that Nano-PCR and LAMP were only sensitive to BPIV3,without cross reaction to other viruses, such as bovine respiratory syncytial virus,infectious bovine rhinotracheitis virus and bovine viral diarrhea virus. In the sensitivity test, Nano-PCR and LAMP showed 10 times sensitivity than that of the traditional PCR technology,with the minimum detection of 4.16×102 copies/μL. Clinical test results showed that the coincidence rate of Nano-PCR and LAMP could reach to 100%, and the positive detection rate was much higher than that of normal PCR.Therefore, the Nano-PCR and LAMP methods established in this study provided a faster, sensitive and reliable tool for the clinical diagnosis of BPIV3. |
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| Keywords: | bovine parainfluenza type-3 virus (BPIV3) Nano-PCR LAMP |
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