Cloning and Functional Bioinformatics Analysis of TSARG7 Gene in Banna Mini-pig Inbred Line

In order to obtain TSATG7 gene sequence and analyze mRNA expression in tissue of Banna Mini-pig inbred line (BMI), we used TSARG7 gene mRNA sequence of pig and related species from GenBank as reference sequences to design specific primers and amplify BMI TSARG7 gene. The semi-quantitative was used to analyze the expression of TSARG7 gene in 10 important tissues, protein sequence was used to carry out functional bioinformatics analysis. A coding sequence of 1 371 bp (GenBank accession No.: KU950831, the corresponding amino acid sequence accession No.: AMY60405) of BMI TSARG7 was obtained, which encoded a protein of 456 amino acids, the protein molecular weight was 52.05 ku, and the isoelectric point was 9.28. Multiple tissue expression indicated that TSARG7 gene expressed highly in the testis, middle and low expression in the other tissues. Functional bioinformatics analysis indicated that TSARG7 protein contained one conserved domain, three transmembrane regions, and no signal peptide sequences;Its N-terminal was hydrophobic and C-terminal was hydrophilic;It had five kinds functional active sites and a 94.1% to located in cytoplasmic. The results of the study would lay a foundation for further study of the gene about its functions in pig spermatogenesis and sexual maturation.