毛棉杜鹃茎段组织培养技术的研究

以毛棉杜鹃嫩茎为试材,采用无菌培养方法,研究了外植体灭菌方法、基本培养基的筛选、增殖培养、伸长培养、生根培养、炼苗与移栽,以期建立毛棉杜鹃组织培养快速繁殖体系。结果表明:毛棉杜鹃嫩茎最佳灭菌方法是用70%酒精浸泡10 s,2%NaClO浸泡15 min,用无菌水冲洗5~6次;WPM培养基为最佳的基本培养基;增殖培养基为WPM+ZT 2.0 mg·L^-1+NAA 0.5 mg·L^-1+蔗糖30 g·L^-1,pH 5.0;伸长壮苗培养基为WPM+ZT 0.5 mg·L^-1+GA32.0 mg·L^-1+蔗糖30 g·L^-1,pH 5.0;生根培养基为WPM+IAA 0.1 mg·L^-1+蔗糖1 g·L^-1,pH 5.0;移栽基质为珍珠岩:草炭土=1∶1配制的培养土,移栽成活率为90%以上。

Study on the Stem in vitro Culture for Rhododendron moulmainense

The stems of Rhododendron moulmainense Hook.f.were used as explants,the method of aseptic culture was adopted and the disinfection of explant,basic medium,proliferation culture,bud elongation culture,rooting and transplanting of seedling were studied,in order to set up the rapid propagation system of Rhododendron moulmainense Hook.f..The results showed that the best explant sterilization methodswas that 70%alcohol for 10 seconds,2%NaClO for 15 minutes,washing with distilled water for 5-6 times.Adventitious bud proliferation culture medium was WPM+ZT2.0 mg·L^-1+NAA 0.5 mg·L^-1+30 g·L^-1 sucrose,pH 5.0,with bud elongation culture medium WPM+ZT 0.5 mg·L^-1+GA32.0 mg·L^-1+30 g·L^-1 sucrose,pH 5.0.The rooting medium was WPM+IAA 0.1 mg·L^-1+1 g·L^-1 sucrose,pH 5.0.The transplanting matrix was perlite∶peat soil=1∶1 with more than 90%transplanting survival rates.