Determination of Suitable Sampling Size in Genetic Diversity Monitoring of Shenxian Pigs' Population

A method was created to reduce the sampling number in order to make the preservation monitoring simpler and accurate for the Shenxian pigs' population. A total of 179 Shenxian pigs as a population was sampled in a certain gradient,each gradient randomly selected 10 times. The genetic diversity indexes of population and sampling groups were calculated using microsatellite primers marker technique. The average and standard deviation of the genetic diversity indexs of 10 groups were compared with that of the selected population, and the minimum sampling size with less changes in genetic diversity was determined as the appropriate sampling scale. The results showed that:For the group with sampling size of 30,the average allele number was 4.6609, the average effective allele number was 3.2325, the average observed heterozygosity was 0.1569, the average expected heterozygosity was 0.6284, the average PIC was 0.5794. The results indicated that the main indexes of genetic diversity in group with sampling size of 30 were similar to those in the whole population, so the sampling size of 30 pigs could be used to represent and instead of large population of Shenxian pigs.