Development of the Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Parvovirus

This study was aimed to develop a simple and rapid method for the porcine parvovirus (PPV) with recombinase polymerase amplification (RPA), which could be a novel and reliable tool for the control and detect of PPV. The RPA was developed using specific primers for the conserved region of PPV VP2 gene. The reaction time was optimized,the RPA reaction could amplify the PPV, and was performed successfully at 38℃ for 30 min in a water bath. The results showed that the detection limit of RPA was 102 copies of plasmid DNA, which was the same as the Real-time quantitative PCR applied in this study, and 100 times more sensitive than conventional PCR. For the clinical samples from the suspected PPV-infected pigs, the positive detection ratio was 82.6% for RPA, which was lower than that of Real-time quantitative PCR (86.9%), but was much higher than conventional PCR (66.7%). The PPV RPA assay developed in the study was simple, rapid and reliable, and was suitable for rapid detection of PPV.