Lithium chloride promotes neuronal differentiation of rat bone marrow mesenchymal stem cells by modulating autophagy |
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Authors: | ZHANG Guang-yu JIA Yan-jie WANG Jun LI San-song TANG Guo-hao WANG Ming-mei WANG Yi-wen ZHU Deng-na |
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Institution: | 1. Department of Children Rehabilitation, The Third Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China;
2. Department of Neurology, The First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, China |
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Abstract: | AIM: To study the influence of lithium chloride (LiCl) on the neuronal differentiation of rat bone marrow mesenchymal stem cells (MSCs), and to explore whether autophagy was involved in this process. METHODS: MSCs were isolated and cultured in vitro. The cells were divided into LiCl group and control group. MSCs were treated with β-mercaptoethanol as an inducer for triggering the cells to differentiate into neurons. The expression of neuronal markers-neuron specific enolase (NSE) and microtubule-associated protein-2 (MAP-2), and autophagic marker-microtubule-associated protein 1 light chain 3 (LC3) were measured by immunofluorescence method and Western blot. An autophagy activator rapamycin and autophagy inhibitor 3-methyladenine (3-MA) were applied to modulate the autophagy in the LiCl treated-cells. The protein expression of NSE and MAP-2 were determined by Western blot. RESULTS: After induction, the expression of NSE and MAP-2 were increased. The percentage of NSE-and MAP-2-positive cells and the expression of NSE and MAP-2 in the LiCl group were greater than those in control group (P<0.05). After induction, the number of LC3-positive dots and the expression of LC3-Ⅱ in LiCl group were greater than those in control group (P<0.05). The expression of NSE and MAP-2 increased when the autophagy was modulated by rapamycin in LiCl treated-cells, and on the contrary, the expression of NSE and MAP-2 were inhibited as autophagy was modulated by 3-MA. CONCLUSION: Lithium chloride may promote the neuronal differentiation of rat bone marrow mesenchymal stem cells by modulating autophagy. |
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Keywords: | Lithium chloride Mesenchymal stem cells Autophagy Neuron Differentiation |
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