Liquid resuscitation combined with hydrogen inhalation reduce acute kidney injury during septic shock in rats

AIM: To investigate the therapeutic effects of a novel fluid resuscitation protocol (early fluid resuscitation plus 2% hydrogen inhalation) on acute kidney injury during septic shock induced by lipopolysaccharide (LPS) in rats.METHODS: Male Wistar rats were randomly divided into 4 groups (15 rats per group):control group, septic shock group, septic shock with early fluid resuscitation group (fluid group) and septic shock with early fluid resuscitation plus 2% hydrogen inhalation group (fluid+H₂ group). The rats were ventilated, and a 2% hydrogen mixture was used in fluid+H₂ group. LPS (10 mg/kg) was administered to establish the septic shock model in rats and fluid resuscitation was performed in fluid group and fluid+H₂ group.RESULTS: Fluid resuscitation with 2% hydrogen inhalation decreased the le-vels of serum creatinine, blood urea nitrogen and neutrophil gelatinase-associated lipocalin. It also reduced oxidative stress injury and decreased renal tumor necrosis factor-α and interleukin-6 levels compared with fluid resuscitation alone.CONCLUSION: Early fluid resuscitation plus 2% hydrogen inhalation provided more protection against acute kidney injury du-ring septic shock.