Effect of Temperature on Bovine Sperm Associated Antigen 11D Gene

To explore the influence of heat stress on the expression level of bovine sperm associated antigen 11D (SPAG11D),which was sperm membrane protein,the mRNA and protein expression levels of SPAG11D were tested in primary bovine testicular cells and sperm. The normal sperm and low activity sperm were collected from Jersey cattle, Bos bubalus and Simmental cattle. The results showed that mRNA and protein expression levels of SPAG11D were highest at 36℃ than others (32, 34, 38 and 40℃) after primary bovine testicular cells cultured for 24 h. And the mRNA and protein expression levels were higher in high temperature groups than low temperature groups. The mRNA expression of SPAG11D in 48 h was consistent with 24 h, but the protein expression levels decreased at high temperature (38 and 40℃). In addition, the protein expression of SPAG11D was lower in low activity sperm than in normal sperm (Jersey cattle and Bos bubalus), and there was no significant change in Simmental cattle sperm.