The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes |
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| Authors: | C G Larsen A O Anderson E Appella J J Oppenheim K Matsushima |
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| Affiliation: | Laboratory of Molecular Immunoregulation, National Cancer Institute, Frederick, MD 21701. |
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| Abstract: | T lymphocyte chemotactic factor (TCF) was purified to homogeneity from the conditioned media of phytohemagglutinin-stimulated human blood mononuclear leukocytes by a sequence of chromatography procedures. The amino-terminal amino acid sequence of the purified TCF showed identity with neutrophil-activating protein (NAP-1). Both TCF and recombinant NAP-1 (rNAP-1) were chemotactic for neutrophils and T lymphocytes in vitro supporting the identity of TCF with NAP-1. Injection of rNAP-1 into lymphatic drainage areas of lymph nodes in Fisher rats caused accelerated emigration of only lymphocytes in high endothelial venules. Intradermal injection of rNAP-1 caused dose-dependent accumulation of neutrophils and lymphocytes. |
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