| 甘薯抗茎线虫病基因的遗传分析及SCAR标记 |
| |
| 引用本文： | 揭琴,姜伟,李华,翟红,马代夫,谢逸萍,刘庆昌. 甘薯抗茎线虫病基因的遗传分析及SCAR标记[J]. 分子植物育种, 2008, 6(3): 523-526 |
| |
| 作者姓名： | 揭琴 姜伟 李华 翟红 马代夫 谢逸萍 刘庆昌 |
| |
| 作者单位： | 中国农业大学;中国农业大学;中国农业大学 |
| |
| 基金项目： | 国家自然科学基金 , 国家高技术研究发展计划(863计划) , 国家科技支撑计划 , 引进国际先进农业科技计划(948计划) |
| |
| 摘 要： | 以甘薯抗茎线虫病品种徐781和感茎线虫病品种徐薯18的杂交F1分离群体的174株单株为材料,对甘薯抗茎线虫病基因的遗传进行了分析。结果表明,徐781的抗茎线虫病为单基因控制,推测其基因型为Rrrrrr,徐薯18的基因型为rrrrrr。采用BSA-RAPD相结合的方法,筛选940条随机引物,发现10条引物在抗、感池间表现多态性。用这10条引物检测两亲本及建池单株,发现只有引物OPP03在8株抗池单株中扩增出一条在8株感池单株中所没有的特异条带,认为该标记与甘薯抗茎线虫病基因连锁。根据该标记对F1代174个单株的扩增结果,利用Mapmaker3.0软件计算遗传距离,表明该标记与抗茎线虫病基因间的遗传距离为14.2cM。将该片断回收、克隆、测序,表明其长度为878bp,该标记命名为OPP03878。根据测序结果,设计1对特异引物,进行特异性扩增,成功地将OPP03878标记转化为SCAR标记,用亲本及分离群体验证表明该分子标记稳定性好。
|
| 关 键 词： | 甘薯(Ipomoea batatas(L.)Lam.) 茎线虫病 RAPD标记 SCAR标记 |
Inheritance Analysis and SCAR Marker of the Gene for Stem Nematode Resistance in Sweetpotato, Ipomoea batatas (L.) Lam. |
| |
| Jie Qin,Jiang Wei,Li Hua,Zhai Hong,Ma Daifu,Xie Yiping,Liu Qingchang. Inheritance Analysis and SCAR Marker of the Gene for Stem Nematode Resistance in Sweetpotato, Ipomoea batatas (L.) Lam.[J]. Molecular Plant Breeding, 2008, 6(3): 523-526 |
| |
| Authors: | Jie Qin Jiang Wei Li Hua Zhai Hong Ma Daifu Xie Yiping Liu Qingchang |
| |
| Affiliation: | Jie Qin 1,2 Jiang Wei 1,2 Li Hua 1,2 Zhai Hong 2,3 Ma Daifu 4 Xie Yiping 4 Liu Qingchang 1,2,31 Key Laboratory of Crop Genomics , Genetic Improvement,Ministry of Agriculture,China Agricultural University,Beijing,100094,2 Beijing Key Laboratory of Crop Genetic Improvement,3 Key Laboratory of Crop Heterosis , Utilization,Ministry of Education,4 Xuzhou Sweetpotato Research Center,Xuzhou,221121 |
| |
| Abstract: | The inheritance of resistance to sweetpotato stem nematode (Ditylenchus destructor) was analyzed in 174 progenies of the F1 single-cross population produced from the cross of resistant parent "Xu781" and susceptible parent "Xushu 18". Single hexasomic inheritance was proposed with genotype of resistant and susceptible parents represented as Rrrrrr and rrrrrr, respectively. Using bulk segregant analysis in combination with RAPD technique, 940 random decamer primers were screened, 10 of them showed polymorphi... |
| |
| Keywords: | |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
