3株猪源绿色气球菌的分离鉴定

为确定云南省不同地区3个猪场仔猪发生非创伤性四肢运动障碍的病原因素,无菌采集病死仔猪深部组织样品,采用血琼脂分离培养病原菌,随后对分离菌株做形态观察、生化试验、药敏试验及16 S rRNA基因测序分析.结果:从3个猪场病料中均分离到菌落及个体形态一致的细菌,其16 S rRNA基因序列与NCBI中7株绿色气球菌参考菌株...

Isolation and identification of 3 strains of Aerococcus viridans from swine

In order to determine the pathogenic agent causing limb disability of piglets from 3 pig farms in different areas of Yunnan Province, the tissue samples from internal organs of dead piglets were aseptically collected for pathogen isolation using blood agar and the resulting bacterial isolates were then subjected to morphological examination, biochemical tests, drug susceptibility tests and sequencing of 16S rRNA gene. Consequently, bacteria obtained from the samples of the 3 pig farms had the same colony and individual morphology, and their 16S rRNA gene sequences shared a higher than 99% homology with 7 reference strains of Aerococcus viridans deposited in NCBI, which proved that the isolates were A. viridans. The 3 isolates were sensitive to clarithromycin, ceftriaxone and penicillin, and resistant to cotrimoxazole, gentamicin and kanamycin. Considering the situation that other pathogen-caused infection was involved in some dead cases and no reports that this bacterium itself had high pathogenicity, the results here indicated that A. viridans could be an important opportunistic bacterium that did harm to the health of pigs.