| 纤维素酶高产菌株的筛选及鉴定 |
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| 引用本文： | 孙海彦,赵平娟,黎绢华,刘恩世,彭明. 纤维素酶高产菌株的筛选及鉴定[J]. 安徽农业科学, 2011, 39(36): 22206-22208 |
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| 作者姓名： | 孙海彦 赵平娟 黎绢华 刘恩世 彭明 |
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| 作者单位： | 中国热带农业科学院生物技术研究所,海南海口571101;海南大学农学院,海南海口571101;中国热带农业科学院生物技术研究所,海南海口,571101 |
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| 基金项目： | 973计划(2010CB126600);863重点计划(2007AA021307);国家自然科学基金(31000029);中国热带农业科学院中央级公益性科研院所基本科研业务费专项(ITBBKF1010,ITB-BZD0951,ITTBB110103);海南省引进集成专项(YJJC2011004);海南省研究生创新基金(Hyb2011-4) |
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| 摘 要： | [目的]从热带雨林土壤中分离纤维素酶高产菌，并对其进行鉴定。[方法]形态观察和18SrDNA序列分析。[结果]筛选到一株纤维素酶高产菌，其形态与里氏木霉很相似，18SrDNA序列与红褐内座茵同源性为99．9％。[结论]筛选到的纤维素酶产生茵为里氏木霉。
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| 关 键 词： | 热带雨林 纤维素酶 里氏木霉 |
Selection and Identification of a Cellulase-producing Strain |
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| Affiliation: | SUN Hai-yan et al (Biotechnology Research Institute,Chinese Academy of Tropical Agriculture,Haikou 571101) |
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| Abstract: | [Objective] This study aimed to identify a cellulase-producer strain which was selected from tropical rain forestry soils.[Method] Morphologic observation and sequence analysis of 18S rDNA were conducted.[Result] A cellulase-producing strain with high activity was obtained,and morphology of the strain was highly similar to that of Trichoderma reesei.Results of sequence analysis showed that the 18S rDNA sequence shared 99% homology with Hypocrea jecorina.[Conclusion] The isolated cellulase-producer strain belonged to Trichoderma reesei. |
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| Keywords: | Tropical rain forestry Cellulase Trichoderma reesei |
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