| 南江黄羊体重体尺性状的RAPD分析研究 |
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| 引用本文： | 张恩平,陈玉林,袁志发,贾正贵,陈瑜.南江黄羊体重体尺性状的RAPD分析研究[J].家畜生态,2005(2). |
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| 作者姓名： | 张恩平 陈玉林 袁志发 贾正贵 陈瑜 |
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| 作者单位： | 西北农林科技大学动物科技学院,西北农林科技大学动物科技学院,西北农林科技大学动物科技学院,四川省南江县南江黄羊研究所,四川省南江县南江黄羊研究所 陕西杨凌 712100,陕西杨凌 712100,陕西杨凌 712100,四川南江 635600,四川南江 635600 |
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| 基金项目： | 国家863计划"优质肉山羊新品种(系)选育及扩繁技术研究"(2002AA242051),教育部科学研究技术研究重点项目"山羊肉用性能候选标记基因SNP的检测与定位"(02076)。 |
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| 摘 要： | 选用4种扩增多态性良好的RAPD引物,对45只南江黄羊基因组DNA进行PCR扩增,对多态标记结果应用SPSS统计之星软件进行统计分析。结果表明:南江黄羊基因组多态频率为84%。RAPD多态标记条带与体重紧密连锁的标记有CY0816引物扩增的ABCF片段和F09引物扩增的ABDF片段,这两种标记组合对体重的贡献率分别为32.6%和28.9%;与体长(A1)、胸围(A3)和胸深(A5)紧密连锁的标记有CY0816引物扩增的ABF片段和F09引物扩增的ABEF片段,其贡献率均在24.2%～28.9%之间。
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| 关 键 词： | RAPD QTLs 体重 体尺 南江黄羊 |
RAPD-based Analysis for the QTLs Related to Body Weight and Body Size of Nanjiang Yellow Goat |
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| ZHANG En-ping,CHEN Yu-lin.RAPD-based Analysis for the QTLs Related to Body Weight and Body Size of Nanjiang Yellow Goat[J].Ecology of Domestic Animal,2005(2). |
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| Authors: | ZHANG En-ping CHEN Yu-lin |
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| Institution: | ZHANG En-ping~1,CHEN Yu-lin~ |
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| Abstract: | The Random Amplified Polymorphic DNA (RAPD) of 45 Nanjiang Yellow Goat was studied by PCR using 4 arbitrary primers. The SPSS Statistics Star Software was used to analyze the relationship between RAPD markers and body weight and body size. The results indicted that the polymorphic frequency of genome of Nanjiang Yellow Goat was 53.49%. The polymorphic markers ABCF of primer CY0816 and ABDF of primer F09 have significant effects on body weight . The contribution ratios of these combinations of markers were 32.6% and 28.9% respectively; The polymorphic markers ABF of primer CY0816 and ABEF of primer F09 have significant effects on body size (body length, chest measurement, chest depth), The contribution ratios were all among of 24.2%~28.9%. |
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| Keywords: | RAPD QTLs body weight body size Nanjiang Yellow Goat |
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