甘露子的组织培养与植株再生

本研究以甘露子嫩茎为材料,进行了愈伤组织的诱导、分化,试管苗的生根、移栽、扦插,以及试管苗移植的研究,并建立起甘露子优良变异植株的无性系,达到了快速繁殖的目的。结果证明:诱导嫩茎愈伤组织的理想培养基是1/2MS+BA0.5～1.0 mg·L-1+2,4-D1.0 mg·L-1;愈伤组织分化的理想培养基是MS+BA1.5mg·L-1+NAA0.1 mg·L-1;生根的理想培养基是1/2MS+IAA 0.2 mg·L-1+NAA 0.1 mg·L-1;以炉灰渣为试管苗的移栽扦插基质,试管苗移栽成活率为97%,扦插成活率为92%。移植的试管苗具有长势非常旺盛,秋天块茎收获量增产18%、根系增加1～2倍、入冬前枯萎时间晚7d的特点。

Studies on Tissue Culture and Plant Regeneration of Stachys siebordii Miq

Callus induction and differentiation were studied using young stem of Stachys siebordii Miq as explant and then the rooting,cutting and transplanting of tube seedling were also studied.The asexual line and rapid propagation technique of Stachys siebordii Miq were established.The results showed that the best medium for callus induction and differentiation of young stem was 1/2MS + 0.5-1.0 mg·L-1 BA + 1.0 mg.L-1 2,4-D and MS + 1.5 mg.L-1 BA + 0.1 mg·L-1 NAA,individually.The ideal medium of rooting was 1/2MS + 0.2mg·L-1 IAA + 0.1 mg·L-1 NAA.With cinder used as the medium,survival percent of 97% for transplantation and 92% for cutting were observed.Transplanted tube seedling was vigorous and yield of cubes increased by 18%.Root system was enlarged by 1-2 times and it wilted 7 days later in the late fall.