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| 磺胺间甲氧嘧啶全抗原的制备研究 | |
| 引用本文： | 张改平,职爱民,瞿明仁,王自良,王选年,李青梅,邢广旭,杨继飞,柴书军,刘庆堂,石团员,宋志超. 磺胺间甲氧嘧啶全抗原的制备研究[J]. 河南农业科学, 2006, 0(3): 96-100 |
| 作者姓名： | 张改平 职爱民 瞿明仁 王自良 王选年 李青梅 邢广旭 杨继飞 柴书军 刘庆堂 石团员 宋志超 |
| 作者单位： | 1. 河南省农业科学院生物技术研究所,河南,郑州,450002 2. 江西农业大学动物科技学院,江西,南昌,330045 3. 河南科技学院动物科学学院,河南,新乡,453003 4. 河南农业大学,河南,郑州,450002 5. 河南省兽药监察所,河南,郑州,450011 |
| 摘 要： | 采用重氮化法，将半抗原磺胺间甲氧嘧啶（SMM）与载体牛血清白蛋白（BSA）和卵清白蛋白（OVA）偶联，制得磺胺间甲氧嘧啶全抗原SMM—BSA和SMM—OVA。通过其紫外扫描光谱、SDS—PAGE凝胶电泳试验，表明半抗原和载体成功偶联，免疫动物以后可以产生满意效价。这另进一步制备抗SMM抗体提供了良好的免疫原。 |
| 关 键 词： | 磺胺间甲氧嘧啶 全抗原 紫外扫描 免疫 鉴定 |
| 文章编号： | 1004-3268（2006）03-0096-05 |
| 收稿时间： | 2005-12-15 |
| 修稿时间： | 2005-12-15 |
Study on Preparation of Complete Antigen of Sulfamonomethoxine |
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| ZHANG Gai-ping,ZHI Ai-min,QU Ming-ren,WANG Zi-liang,WANG Xuan-nian,LI Qing-mei,XING Guang-xu,YANG Ji-fei,CHAI Shu-jun,LIU Qing-tang,SHI Tuan-yuan,SONG Zhi-chao. Study on Preparation of Complete Antigen of Sulfamonomethoxine[J]. Journal of Henan Agricultural Sciences, 2006, 0(3): 96-100 | |
| Authors: | ZHANG Gai-ping ZHI Ai-min QU Ming-ren WANG Zi-liang WANG Xuan-nian LI Qing-mei XING Guang-xu YANG Ji-fei CHAI Shu-jun LIU Qing-tang SHI Tuan-yuan SONG Zhi-chao |
| Affiliation: | 1. Institute of Biology and Technique Science.s, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 2. College of Animal Sciences,Jiangxi Agricultural University, Nanchang 330045 ,China; 3. College of Animal Science, Henan Institute of Science and Technology,Xinxiang 453003, China; 4. Henan Agricultural University, Zhengzhou 450002, China; 5. Henan Institute of Veterinary Drug Control, Zhengzhou 450002, China |
| Abstract: | Semiantigen sulfamonomethoxine(SMM) was coupled with carrier protein bovine serum albumin(BSA) and ovalbumin to prepare complete antigen SMM-BSA and SMM-OVA by the method of diazotization.UV scanning spectrum,SDS-PAGE and animal immunity all indicated that the complete antigens both SMM-BSA and SMM-OVA were successfully produced.The present research supplied an excellent immune antigen for further preparation of antibody to SMM. |
| Keywords: | Sulfamethoxydiazine Complete antigen UV scanning spectrum Immunity Identify |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |