| 石斛兰品种遗传变异的RAPD检测 |
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| 引用本文： | 罗远华,余志金,莫光武,梁定栽,莫饶.石斛兰品种遗传变异的RAPD检测[J].安徽农业科学,2009,37(25). |
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| 作者姓名： | 罗远华 余志金 莫光武 梁定栽 莫饶 |
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| 作者单位： | 海南大学农学院,海南儋州,571737;海南省林业总公司,海南海口,570203 |
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| 基金项目： | 国家科技支撑计划项目 |
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| 摘 要： | 目的]利用RAPD技术检测石斛兰品种的遗传变异性。方法]以4个母本园石斛兰品种和1个试管苗品种为材料,经DNA提取后,采用10 bp随机引物进行RAPD检测其遗传变异性。结果]绿意(Den.Burana Green)和粉红钻石(Den.Pink Thamond)2个母本园品种未发现变异,熊猫(Den.BigPanda)和泰国白(Den.Thailand White)2个母本园品种变异率分别为3.3%和23.3%;红索尼娅(Den.Red Sonia)试管苗变异率为10.9%。结论]建立了石斛兰品种遗传变异的RAPD检测技术。
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| 关 键 词： | 石斛兰 品种 试管苗 RAPD 遗传变异 |
Study on the Genetic Variation of Dendrobium by RAPD Molecular Markers |
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| LUO Yuan-hua et al.Study on the Genetic Variation of Dendrobium by RAPD Molecular Markers[J].Journal of Anhui Agricultural Sciences,2009,37(25). |
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| Authors: | LUO Yuan-hua |
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| Institution: | LUO Yuan-hua et al (College of Agronomy,Hainan University,Danzhou,Hainan 571737) |
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| Abstract: | Objective] The aim of research was to detect the genetic variation of Dendrobium by RAPD molecular marker.Method] With 4 different varieties of Dendrobium and 1 variety in vitro as materials,after DNA extraction,10 bp random primers was used for RAPD detection of genetic variation.Result]Burana Greenand Pink Thamond 2 varieties not found genetic variation;the genetic variation rate of BigPanda was 3.3%,the genetic variation rate of Thailand White was 23.3%;the genetic variation rate of Red Sonia in vitro... |
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| Keywords: | RAPD |
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