ELISA技术在畜产品安全检测中的应用

酶联免疫吸附试验 (Enzyme-L inkedImmunosorbent Assay,EL SA)主要是将抗原(或抗体 )吸附于固相载体 ,在载体上进行免疫酶染色 ,最后底物显色用肉眼或分光光度计判定结果的一种检测方法。随着生活水平的不断提高 ,人们越来越关注食品中各种有害物质对健康的影响。对食品中各种有害物质的监测显得越来越重要。畜产品作为食品的重要组成部分 ,其安全检测也不容忽视。检测畜产品中有害物质的方法很多 ,大多数需要昂贵的仪器设备 ,且操作繁琐 ,成本较高。酶联免疫吸附法因其具有操作简便、快速准确等优点 ,成为畜产品中药物残留、毒素、农药、微生物检测中最重要的检测技术之一 ,得到人们的青睐 ,展示了很好的应用前景

The Application of ELISA in the Analysis of Livestock Product Safety

Enzyme-Linked Immunosorbent Assay (ELISA) is a method that combines enzymes' high efficiency of catalyzing and the specific immune reaction between antigen and antibody. Antibody(or antigen) is first bound to a solid surface; then immunoenzyme converts a colorless substance to a colored product, which could be detected by spectrometer. With increasing standards of human lives, more and more concerns are given to some possible harmful materials in food. Thus to detect such health-risking materials becomes more and more important. Therefore, safety determination of animal products, one important component of food, need high attention. Though many method for the detection exist, most of them need expensive instruments, high prices, and troublesome operations. Due to its convenient operation, quickness and accuracy, ELISA have become one of the most important technology used to detect harmful materials in animal products, such as residues of drugs, toxins, pesticides, microbes, etc. This technology is being favored by many people and it shows a bright prospects.