日本紫色甘薯常见病毒病的检测

采用硝化纤维素膜酶联免疫吸附检测法 (NCM -ELISA)和症状诊断法 ,对从日本引进的紫色甘薯病毒病发生情况进行检测。NCM -ELISA检测结果表明 ,从日本引进的紫色甘薯分别感染了甘薯羽状斑驳病毒 (SPFMV)、甘薯轻度斑驳花叶病毒 (SPMMV)、甘薯褪绿斑点病毒 (SPCFV)、甘薯潜隐病毒 (SPLV)、C - 6病毒和C - 8病毒。其中 ,SPFMV和SPMMV感染最普遍 ,SPCFV和SPLV次之 ,而C - 6和C - 8感染较少。在供试的 14个日本紫色甘薯品种 (系 )中 ,A4、A5和山川紫等 3个品系 (种 )感染上述全部 6种病毒 ,其余 11个甘薯品系只是感染了其中几种病毒 ;症状观察结果表明 ,感染病毒的紫色甘薯叶片出现明显的症状 ,主要表现为褪绿斑驳、畸形、坏死、变色等 4种类型。

Virus Detection on Purple Sweet Potato Introduced from Japan

The virus infection in 14 strains or cultivars of purple sweet potato from Japan was surveyed by the method of enzyme linked immunosorbent assay on nitrocellulose membranes (NCM-ELISA) and symptomatology. The results of NCM-ELISA showed that all of strains or cultivars of purple sweet potato were infected by one or more of the following viruses: sweet potato feather mottled virus (SPFMV),sweet potato mild mottled virus (SPMMV),sweet potato chlorotic fleck virus(SPCFV),sweet potato latent virus (SPLV), C-6 and C-8,in which;the strains or cultivars of A4, A5 and “Shanchuanzi” were infected by the six viruses metioned above. Meanwhile, the results of symptomatology showed that the major symptoms in leaves of purple sweet potato infected by virus were four types: chlorotic fleck, deformation, necrosis and changing color.