| 响应面法优化北虫草产多糖液体发酵培养基 |
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| 引用本文： | 王飞,刘霞,陈明辉. 响应面法优化北虫草产多糖液体发酵培养基[J]. 安徽农业科学, 2007, 35(8): 2218-2218,2224. DOI: 10.3969/j.issn.0517-6611.2007.08.009 |
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| 作者姓名： | 王飞 刘霞 陈明辉 |
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| 作者单位： | 江西农业大学生物科学与工程学院,江西南昌,330045;南昌理工学院生物环境工程系,江西南昌,330013 |
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| 基金项目： | 江西农业大学青年科学基金项目. |
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| 摘 要： | 采用响应面法(RSM)对北虫草(Codyceps militaris(L.)Link.)液体发酵培养基的3种主要成分蔗糖、蛋白胨和KH2PO4进行优化,采用多元二次回归方程拟合3种因素与多糖产量之间的函数关系.通过岭脊分析,获得培养基中3因素最佳浓度为:蔗糖3.23%,蛋白胨1.05%,KH2PO40.08%,培养液多糖含量为1.977 970×10-2g/ml.
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| 关 键 词： | 响应面法 北虫草 培养基 岭脊分析 |
| 文章编号： | 0517-6611（2007）08-02218-01 |
| 修稿时间： | 2006-12-18 |
Study on Liquid Fermentation Medium for Codyceps militaris by Response Surface Method |
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| WANG Fei ,et al. Study on Liquid Fermentation Medium for Codyceps militaris by Response Surface Method[J]. Journal of Anhui Agricultural Sciences, 2007, 35(8): 2218-2218,2224. DOI: 10.3969/j.issn.0517-6611.2007.08.009 |
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| Authors: | WANG Fei et al |
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| Affiliation: | College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, Jiangxi 330045 |
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| Abstract: | Three major components(saccharose,tryptone and KH2PO4) of liquid fermentation medium for Codyceps militaris(L.)Link were optimized by response surface method(RSM).The method of fitting a regression equation to functional relation between the three components and polysaccharide yield was used.Optimal concentration of the three components was obtained according to ridge analysis,which were 3.23 % saccharose,1.05 % tryptone,0.08 % KH2PO4.And polysaccharide content in liquid fermentation medium was 1.975×10-2 g/mL. |
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| Keywords: | Response surface method Codyceps militaris(L.) Link Culture medium Ridge analysis |
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