Cloning and expression analysis of HXK1 gene from Jatropha curcas L. |
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| Institution: | 1. Key Laboratory of Biodiversity Conservation in Southwest, State Forestry Administration, Southwest Forestry University, Kunming, China;2. Center for Yunnan Plateau Biological Resources Protection and Utilization, Qujing Normal University, Qujing, China;3. Key Laboratory of Yunnan Province Universities of the Diversity and Ecological Adaptive Evolution for Animals and Plants on Yunnan-Guizhou Plateau, Qujing Normal University, Qujing, China |
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| Abstract: | HXK (Hexokinase) gene family and the role of JcHXK1 in Jatropha curcas L. were explored. Totally 4 HXK genes JcHXK1, JcHXK2, JcHXK3 and JcHKL1 were identified and complete ORF of JcHXK1 was cloned. Functional domain, phylogenetic evolution and low-temperature expression characteristics were analyzed. Results showed that full-length JcHXK1 cDNA was 1497 bp, encoding 498 amino acids with molecular weight of 53.81 ?kDa and pI of 5.03. Further phylogenetic evolutionary analysis demonstrated HXK1 protein was clustered into 6 small branches and 2 large branches. Sequence alignment showed that HXK1 contained several conserved glycine residues and hydrophobic channels. Prokaryotic expression vector of JcHXK1 was constructed and 12% SDS-PAGE detection showed that it was highly expressed in E. coli. These research was expected to lay a foundation for further gene functional verification and cold signal transduction mechanism for HXK1 in Jatropha curcas L. |
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| Keywords: | Resilience Gene cloning Functional analysis |
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