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| 樱桃ACC氧化酶基因的克隆和序列分析 | |
| 引用本文： | 王俊英,张开春,王俊平,张军科.樱桃ACC氧化酶基因的克隆和序列分析[J].华北农学报,2002,17(1):11-15. |
| 作者姓名： | 王俊英 张开春 王俊平 张军科 |
| 作者单位： | 1. 北京林业大学,生物学院,北京,100083 2. 北京市农林科学院林果所,北京,100093 3. 中国农业大学,作物学院,北京,100094 4. 西北农林科技大学,园艺系,陕西,杨凌,712100 |
| 摘 要： | 以樱桃基因组DNA为模板定向克隆其ACC氧化酶基因。对PCR扩增的目的片段用1％琼脂糖凝胶电泳检测后，进行回收、连续转化，通过蓝白筛选挑取白色菌落提取质粒DNA进行酶切鉴定，确定重组子后采用双脱氧终止法测定DNA全序列。DNA测序结果显示，樱桃的ACC氧化酶基因序列全长为1310bp，由2个外显子和3个内含子构成，外显子总长为1000bp。同源性分析可知，樱桃与桃的ACC氧化酶基因DNA序列同源性达97．8％。 |
| 关 键 词： | 樱桃 ACC氧化酶 基因克隆 序列分析 乙烯 生物合成 果实成熟 |
| 文章编号： | 1000-7091(2002)01-0011-05 |
| 修稿时间： | 2001年10月26 |
Cloning and Sequencing of ACC Oxidase Genes in Cherry Fruit and Leaf |
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| WANG Jun ying ,ZHANG Kai chun ,WANG Jun ping ,ZHANG Jun ke.Cloning and Sequencing of ACC Oxidase Genes in Cherry Fruit and Leaf[J].Acta Agriculturae Boreali-Sinica,2002,17(1):11-15. | |
| Authors: | WANG Jun ying ZHANG Kai chun WANG Jun ping ZHANG Jun ke |
| Institution: | WANG Jun ying 1,ZHANG Kai chun 2,WANG Jun ping 3,ZHANG Jun ke 4 |
| Abstract: | The ACC oxidase gene was cloned from genomic DNA of prunus pseudocerasus L.Destination segments amplified by PCR were reclaimed,connected and transformed after examination by 1% agarose gel electrophoresis.By blue white screening,the plasmid DNA extracted from white colony was identified using restriction enzyme analysis and the DNA sequence was determined following recon determination.The results of DNA sequencing revealed that the ACC oxidase gene sequence of Prunus pseudocerasus L was 1 310 bp and consisted of 2 exons and 3 introns and the full length of the exons was 1 000 bp.Homogenous analysis indicated that homologue of DNA sequence of the ACC oxidase gene in Prunus pseudocerasus L and Prunus persica Batsch reached 97 8%. |
| Keywords: | Cherry ACC oxidase Gene cloning DNA sequencing |
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