| 溴氰菊酯残留检测ELISA试剂盒的研制 |
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| 引用本文： | 张婧,王春娜,魏朝俊,赵建庄,史亚俊.溴氰菊酯残留检测ELISA试剂盒的研制[J].北京农学院学报,2009,24(2):27-30. |
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| 作者姓名： | 张婧 王春娜 魏朝俊 赵建庄 史亚俊 |
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| 作者单位： | 1. 北京农学院植物科学技术学院,北京,102206
2. 北京农学院基础教学部,北京,102206 |
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| 基金项目： | 北京市自然科学基金，北京市教委资助项目，北京市教委农产品储藏与加工重点建设学科资助项目，北京市教委果树学重点学科资助项目 |
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| 摘 要： | 在前期建立溴氰菊酯间接竞争ELISA检测方法的基础上，研制组装出溴氰菊酯残留检测ELISA试剂盒，并以苹果为试样，对该试剂盒的灵敏度、精密度、准确度、特异性进行参数测定。本试剂盒的IC50为3．999-7．709μg／mL，检出限为0．011-0．024mg／kg，检测范围为0．015～100μg／mL，重现性较好，特异性强，以0．1，0．5，1．0mg／kg3个水平添加苹果，回收率为86．2％～105．8％，变异系数为6．0％～9．8％，接近气相色谱法的检测水平，可满足溴氰菊酯残留初筛检测需要。该试剂盒的成功研制为实际样品检测提供理论基础。
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| 关 键 词： | 溴氰菊酯 ELISA 试剂盒 |
Development of ELISA Kit for Deltamethrin Residue |
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| ZHANG Jing,WANG Chun-na,WEI Chao-jun,ZHAO Jian-zhuang,SHI Ya-jun.Development of ELISA Kit for Deltamethrin Residue[J].Journal of Beijing Agricultural College,2009,24(2):27-30. |
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| Authors: | ZHANG Jing WANG Chun-na WEI Chao-jun ZHAO Jian-zhuang SHI Ya-jun |
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| Institution: | a.Department of Plant Science and Technolog;b.Department of Basic Sciences;Beijing University of Agriculture;Beijing 102206;China |
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| Abstract: | On the base of IC-ELISA established,the sensitivity,precision,accuracy and specific of the kit were tested on apple samples.The technique parameters were as follows: IC50 ranged from 3.999 μg/mL to 7.709 μg/mL;the limits of detection ranged from 0.011 mg/kg to 0.024 mg/kg,the detection range was from 0.015μg/mL to 100 μg/mL.When the spiked concentrations of deltamethrin were 0.1 mg/kg,0.5 mg/kg and 1.0 mg/kg in apple,the recovery of deltamethrin was 86.2%~105.8% with coefficients of variation of 6.0%~9.8%.The results were close to gas chromatographic(GC) detection. |
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| Keywords: | ELISA |
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