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Identification of Renieramycin A as an Antileishmanial Substance in a Marine Sponge Neopetrosia sp. |
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| Authors: | Yoichi Nakao Takeru Shiroiwa Shuhei Murayama Shigeki Matsunaga Yasuyuki Goto Yoshitsugu Matsumoto Nobuhiro Fusetani |
| Affiliation: | 1.Laboratory of Aquatic Natural Products Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan;2.Department of Molecular Immunology, Graduate School of Agricultural and life Sciences, The University of Tokyo, Tokyo 113-8657, Japan |
| Abstract: | The newly developed assay system using recombinant Leishmania amazonensis expressing enhanced green fluorescent protein (La/egfp) has been applied to the screening of Japanese marine sponges for antileishmanial activity. Bioassay-guided fractionation of an active sponge Neopetrosia sp. afforded an active compound which was identified as renieramycin A by spectroscopic analysis. It inhibited La/egfp with an IC50 value of 0.2 μg/mL. |
| Keywords: | leishmaniasis marine sponge renieramycin A |