Isolation and characterization of Indian isolates of camel pox virus |
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| Authors: | V. Bhanuprakash V. Balamurugan M. Hosamani G. Venkatesan Bina Chauhan V. A. Srinivasan R. S. Chauhan K. M. L. Pathak Raj Kumar Singh |
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| Affiliation: | (1) Pox Virus Diseases Laboratory, Division of Virology, Indian Veterinary Research Institute, Mukteswar, 263138 Nainital, Uttarakhand, India;(2) Division of Virology, Indian Veterinary Research Institute, Mukteswar, 263138 Nainital, Uttarakhand, India;(3) Project Directorate on Animal Disease Monitoring and Surveillance (PD_ADMAS), Hebbal, Bangalore, 560 024, Karnataka, India;(4) Indian Veterinary Research Institute, Bangalore, 560024, Karnataka, India;(5) Division of Biological Products, Indian Veterinary Research Institute, Izatnagar, 243122, India;(6) Indian Immunologicals Ltd., Rakshapuram, Gachibowli, Hyderabad, 500032, Andhra Pradesh, India;(7) Indian Veterinary Research Institute, Deemed University, Izatnagar, 243 122, Uttar Pradesh, India;(8) National Research Centre on Camel, Jorbeer, Bikaner, Rajasthan, India;(9) National Research Centre on Equines, Sirsa Road, Hisar, 125001, Haryana, India; |
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| Abstract: | In this study, we isolated and identified three camel pox viruses (CMLV) from two outbreaks of camel pox infection in camels associated with eruptions on cheeks, nostrils, limbs, scrotum, and sheath that occurred at different places of Bikaner district, Rajasthan (India). The scab specimens collected were subjected for virus isolation in Vero cell culture, and the isolated viruses were characterized by employing polymerase chain reaction (PCR) and sequencing. The causative agent was identified as CMLV, based on A-type inclusion, B5R and C18L genes-specific PCRs and partial sequencing of these genes, which clearly confirmed that the outbreaks were caused by CMLV and identity of CMLV isolates. Further, phylogenetic analysis of partial C18L gene sequences have showed that Indian CMLV are clustered together with other reported isolates/strains. |
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