The Zea mays b-32 ribosome-inactivating protein efficiently inhibits growth of Fusarium verticillioides on leaf pieces in vitro |
| |
Authors: | Chiara Lanzanova Maria Gabriella Giuffrida Mario Motto Cristina Baro Guenter Donn Hans Hartings Elisabetta Lupotto Maria Careri Lisa Elviri Carlotta Balconi |
| |
Affiliation: | (1) C.R.A., MAC, Unità di Ricerca per la Maiscoltura, via Stezzano 24, 24126 Bergamo, Italy;(2) ISPA-CNR c/o Bioindustry Park del Canavese, via Ribes 5, 10010 Collaretto Giacosa (TO), Italy;(3) Bayer CropScience, D-65926 Frankfurt am Main, Germany;(4) Dipartimento di Chimica Generale ed Inorganica, Chimica Analitica, Chimica Fisica, Università degli Studi di Parma, Parco Area delle Scienze 17/A, 43100 Parma, Italy |
| |
Abstract: | In maize endosperm, a cytosolic albumin, b-32, with a molecular weight of 32 kDa is synthesised in temporal and quantitative coordination with the deposition of storage proteins. This protein has homology with several previously characterised Ribosome-Inactivating Proteins (RIPs). To verify if the maize plant expressing b-32 in various tissues has an increased tolerance to fungal pathogens, transgenic plants were obtained through genetic transformation using a chimeric gene containing the b-32 coding sequence downstream of a constitutive 35SCaMV promoter. A set of four independent homozygous progenies expressing b-32, were selected for a detailed analysis of b-32 expression in leaves and for pathogenicity tests. A differential b-32 content in leaf protein extracts was recorded in the transgenic progenies. Proteomic investigations on protein leaf extracts were carried out; the overlapping of the two-dimensional electrophoresis maps demonstrated the presence in a transgenic progeny, of additional spots, identified as b-32 and as a protein for herbicide resistance, in comparison to the negative control. Transgenic progenies were tested in bioassays to evaluate the response to Fusarium attack in leaf tissues. Preliminary experiments supported the choice of bioassay parameters for a reliable evaluation of transgenic progenies. The negative control was most susceptible to Fusarium verticillioides attack, compared to transgenic progenies. The data obtained indicate that maize b-32 was an effective antifungal protein by reducing Fusarium infection progression. Additionally, the reduction in Fusarium attack symptoms was related to b-32 concentration in leaf tissues. |
| |
Keywords: | Antifungal protein Maize Plant defence Transgenic plant |
本文献已被 SpringerLink 等数据库收录! |
|