| tPA乳腺组织特异性表达载体构建及转基因小鼠建立 |
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| 引用本文： | 赵宝全,张守峰,赵君,扈荣良,章金钢,殷震.tPA乳腺组织特异性表达载体构建及转基因小鼠建立[J].中国兽医学报,2002,22(2):125-127. |
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| 作者姓名： | 赵宝全 张守峰 赵君 扈荣良 章金钢 殷震 |
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| 作者单位： | 解放军军需大学军事兽医研究所,吉林,长春,130062 |
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| 基金项目： | 军队医药卫生基金资助课题 ( 96 Z0 5 0 ) |
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| 摘 要： | 以pKB、pcDNA3和pCPA为原始质粒，构建BLG启动子调控的组织型纤溶酶原激活剂（tPA）乳腺组织特异性表达载体pBTA。以SalⅠ酶切质粒pBTA，回收5.45kb基因片段BLG－tPA-bGHpolyA，通过显微注射，导入昆明小鼠受精卵的雄原核内。共注1365枚，将存活的1053枚移植的50只同期发情假孕母鼠的输卵管内，怀孕24只，共产仔79只，上述仔鼠通过PCR检测，结果19只阳性。Southern blotting检测上述19份PCR阳性小鼠基因组，其中4只为整合阳性，说明已获得了乳球蛋白启动子调控下的tPA转基因小鼠，为tPA在小鼠体内表达研究提供了必要条件。
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| 关 键 词： | tPA 转基因 小鼠 乳腺生物反应器 乳腺组织特异性表达载体 溶栓药物 |
| 文章编号： | 1005-4545(2002)02-0125-02 |
| 修稿时间： | 2000年9月18日 |
Construction of a Human Tissue-type Plasminogen Activator Tissue Specific Expression Vector and Establishment of Its Transgenic Mice |
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| ZHAO Bao-quan,ZHANG Shou-feng,ZHAO Jun,HU Rong-liang ,ZHANG Jin-gang,YIN Zhen.Construction of a Human Tissue-type Plasminogen Activator Tissue Specific Expression Vector and Establishment of Its Transgenic Mice[J].Chinese Journal of Veterinary Science,2002,22(2):125-127. |
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| Authors: | ZHAO Bao-quan ZHANG Shou-feng ZHAO Jun HU Rong-liang ZHANG Jin-gang YIN Zhen |
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| Institution: | ZHAO Bao-quan,ZHANG Shou-feng,ZHAO Jun,HU Rong-liang *,ZHANG Jin-gang,YIN Zhen |
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| Abstract: | Tissue-type plasminogen activator cDNA was cloned on downstream of the bovine beta-lactoglobulin promoter to form a expression vector pBTA. A 5.45kb fragment containing the tPA expression cassette was recovered and purified for microinjection. The microinjected ova were transferred to 50 pseudopregnant mouse recipients. 79 offspring were born. 19 of them were proved to be positive of gene integration by PCR, 4 mice were further confirmed to be transgenic by Southern hybridization. |
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| Keywords: | tPA transgenic mice gene integration |
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