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一株分离自葛藤根际高效溶磷细菌特性研究及菌株鉴定
引用本文:李显刚,姚拓,王小利,陆瑞霞.一株分离自葛藤根际高效溶磷细菌特性研究及菌株鉴定[J].中国土壤与肥料,2012(2):87-91.
作者姓名:李显刚  姚拓  王小利  陆瑞霞
作者单位:1. 甘肃农业大学草业学院,甘肃兰州,730070
2. 甘肃农业大学草业学院,甘肃兰州730070;草业生态系统教育部重点实验室,甘肃兰州730070;中-美草地畜牧业可持续发展研究中心,甘肃兰州730070
3. 贵州省草业研究所,贵州 贵阳,550006
基金项目:国家自然基金(30960265);贵州省农业攻关项目[黔科合NY字(2009)3067];贵州省社会发展攻关项目[黔科合NY字(2011)3104号]
摘    要:对分离自葛藤(Pueraria lobata L.)根际的一株高效溶磷细菌GTR15进行促生特性、主要生理生化指标测定和16S rDNA序列分析。结果表明,菌株GTR15的HD/CD值(溶磷圈直径HD,菌落直径CD)为2.22,28℃液体振荡培养7 d后对磷酸钙的溶解量为138.72 mg.L-1,分泌IAA(3-吲哚乙酸)及有机酸量分别为14.44 mg.L-1、46.00 mmol.L-1。菌株革兰氏染色为阴性,细胞短杆状;淀粉水解、吲哚、V-P(二乙酰试验)、苯丙氨酸脱氨酶、明胶液化及M-R(甲基红)试验呈阴性;柠檬酸盐、过氧化氢酶、硫化氢及硝酸盐还原等试验呈阳性,结合菌株16S rDNA序列分析结果,初步鉴定为肠杆菌(Enterobacter sp.)。该菌株在研制高效微生物磷肥接种剂方面可能具有较大潜力。

关 键 词:溶磷菌  溶磷能力  16SrDNA  分泌IAA  葛藤
收稿时间:2011/8/30 0:00:00
修稿时间:2011/10/26 0:00:00

Characteristics and identification of a dissolving phosphate bacteria strain isolated from rhizosphere of Pueraria lobata
LI Xian-gang , YAO Tuo , WANG Xiao-li , LU Rui-xia.Characteristics and identification of a dissolving phosphate bacteria strain isolated from rhizosphere of Pueraria lobata[J].Soil and Fertilizer Sciences in China,2012(2):87-91.
Authors:LI Xian-gang  YAO Tuo  WANG Xiao-li  LU Rui-xia
Institution:1.Pratacultural College,Gansu Agricultural University,Lanzhou 730070;2.Key Laboratory of Grassland Ecosystem,Ministry of Education,Lanzhou 730070;3.Sino-U.S.Centers for Sustainable Development of Grassland and Animal Husbandry,Lanzhou 730070;4.Guizhou Institute of Prataculture,Guiyang 550006)
Abstract:Phosphate-dissolving bacteria GTR15 was isolated from the rhizosphere of Pueraria lobata L.The characteristics,including phosphate solubilizing,produing IAA(indole-3-acetic acid)and organic acid and physiological characteristics and 16S rDNA sequence analysis of bacteria were determined.The results showed that the HD/CD value of GTR15 strain was 2.22,when it was inoculated into PKO liquid culture,the release of maximal available soluble phosphorus reached to 138.72 mg·L-1 after 7 days incubation at 28℃.It had the maximal capacity of producing IAA(14.44 mg·L-1)and organic acid(46.00 mmol·L-1).Strain GTR15 was identified as Enterobacter sp.,based on its physiological characteristicsGram stain negative,short rod-shaped cells;The tests which including starch hydrolysis,indole,V-P(Diacetate),phenylalanine deaminase,gelatin liquefaction and M-R(Methyl red)were negative,and the tests which including citrate,catalase,hydrogen sulfide and nitrate reduction were positive.]and 16S rDNA sequence analysis.The strain has the great potential for developing the efficient microbial phosphate inoculant.
Keywords:phosphate-dissolving bacteria  capacity of phosphate solubilization  16S rDNA  producing IAA  Pueraria lobata
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